ABSTRACT
Renal osteodystrophy is a multifactorial disorder of bone remodelling that develops in patients with chronic renal failure [CRF]. Biochemical markers of bone turnover have been proposed for the noninvasive diagnosis of renal osteodystrophy. The purpose of this study was to evaluate intact parathyroid hormone [iPTH] and some markers of bone disease in predialysis [preD] and haemodialysis [HD] CRF patients and correlate them with bone mineral density [BMD]. Several biochemical markers were measured in the serum of 74 CRF patients [38 preD patients and 36 patients on regular HD]. In addition, 30 healthy volunteers were included as controls. BMD of all patients was measured by means of calcaneal ultrasonography. BMD was measured by means of ultrasound. BMD was significantly decreased in both patient groups when compared with controls. Also, it was significantly lower in patients with osteoporosis than in those with osteopenia. iPTH, total alkaline phosphatase [ALP] and osteocalcin [OC] levels were significantly elevated in both patient groups when compared with controls. Ionized calcium [Ca[2+]], free carnitine and insulin-like growth factor-1 [IGF-1] levels were significantly decreased in patients compared with controls. There was a significant inverse correlation of BMD with iPTH, ALP and OC and a significant positive correlation with Ca[2+] and IGF-1 in HD patients. PreD patients showed significant inverse correlation of BMD with iPTH and ALP and significant positive correlation with Ca[2+]. The results of the present study suggested that ultrasound is a useful method for evaluating BMD and provides information about diverse regional skeletal changes in CRF patients. iPTH, ALP, OC and Ca[2+] can predict renal osteodystrophy in preD and HD CRF patients. PreD and HD CRF patients often have low serum concentrations of free carnitine and IGF-1
ABSTRACT
Adipose tissue secretes a large number of adipocytokines such as leptin, resistin, and adiponectin. Many of these hormones and cytokines are altered in obese individuals and may lead to disruption of the normal balance between cell proliferation, differentiation, and apoptosis. The aim of our work was to investigate the disturbance of secretion of adiponectin and resistin in de novo and relapsed acute lymphoblastic leukemia [ALL] in Egyptian children and determine whether adiponectin and resistin are implicated in increased risk relapse compared to healthy individuals. Measurements of adiponectin and resistin were performed at diagnosis, in 32 patients with de novo ALL aged 3 to 18 years [mean 9.8 y] and 19 children with relapsed ALL aged 5 to 17 [mean 9.9 yr]. 10 apparently healthy children with matched age and sex were used as controls. Mean adiponectin levels were low [P < 0.05], whereas mean resistin levels were high [P<0.05] at diagnosis and relapsed ALL [compared to healthy controls]. A significant decrease of adiponectin levels was observed in relapsed ALL compared to de novo ALL. In contrast resistin was significantly increased in relapsed ALL compared to de novo patients. Adiponectin in ALL subjects inversely correlated with resistin level [r = -0.51, P < 0.001]. Low adiponectin and high resistin level at diagnosis suggest their implication in ALL pathogenesis and may serve as potential clinically significant diagnostic markers to detect leukemic relapse
Subject(s)
Humans , Female , Male , Adiponectin/blood , Resistin/blood , Leptin/blood , Biomarkers, Tumor/blood , Cell Proliferation , RecurrenceABSTRACT
The aim of this study was to detect the prognostic significance of survivin level and the expression of total p53 in acute lymphoblastic leukemia [ALL] and its correlation to patients' outcome. Sixty two children newly diagnosed with acute lymphoblastic leukemia were treated with chemotherapy and followed up for 2 years or until death. Twenty apparently healthy volunteers with matched age and sex were taken as control. Survivin protein was measured by quantitative sandwich enzyme immunoassay and total human p53 was measured by Flow cytometry in peripheral blood at diagnosis and at complete remission. A highly significant elevation [P<0.0001] was found in survivin protein and total p53 levels in acute lymphoblastic leukemia children patients at diagnosis compared to controls. At complete remission a significant decrease of the two indices were found in ALL patients compared to those at diagnosis [P<0.000l]. Survivin protein and total p53 was significantly higher in non-survived compared to survived group [P<0.0001 and /M].016, respectively]. A positive correlation was found between survivin level and total human p53 level in children with ALL 0-0.501 and P<0.0001]. survivin protein is related to anti-apoptotic proteins and its high expression lead to unsuccessful treatment of ALL. Survivin and TP53 are new prognostic tools in ALL, independent of age and sex